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KMID : 0368019900130010117
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Journal of Soonchunhyang University
1990 Volume.13 No. 1 p.117 ~ p.128
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A Study on the Diagnostic Technique for Clonorchiasis by EITB
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Abstract
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The experiments on Enzyme-linked Immunoelectro Transfer Blot(EITB) of the Clonorchis sinensis antigen and infected sera were carried out to find the specific antigenic component of the Clonorchis sinensis, and observe the antibody binding bands of the Clonorchis sinensis crude antigen(CAA) with clonorchiasis antisera as well as the cross immune reaction with paragonimiasis antisera.
From the result of EITB between the clonorchiasis antisera and CCA, 14 antibody binding bands have came out. All of above, stronger antibody binding bands were ranged in molecular weight (MW) 45, 000, 42, 000, 38, 000, 34, 500, and 20, 000 Balton. It was also consistent in major bands on SDS-PAGE of ´CCA. There was qualitative and quantitative difference of antibody in worm burden by EPG(egg per gram). In EITB between CCA and paragonimiasis antisera, there was the cross immune reaction in the range of MW 45,000, 42,000 32, 000 and 20,00o dalton.
The nine bands were appeared in EITB of CAA, and the MW of each band was consistent in the case of CCA. Also, the cross immune reaction with paragonimiasis antisera was occurred in the range of MW 45, 000, 42, 000, 32,000 and 20, 000 dalton.
In conclusion, it seemed that the antigenic bands with specific reaction on clonorchiasis were in the range of MW 38, 000 dalton and 30, 000~25, 000 dalton, but especially MW 38,000 dalton.
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